2007-01-23 · Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.

The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8).

Molecular Weight: Observed Mol.Wt.: 70kD. Ku is a dimeric protein complex that binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.Ku is evolutionarily conserved from bacteria to humans. The ancestral bacterial Ku is a homodimer (two copies of the same protein bound to each other). Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80.

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This domain only makes a small contribution to the dimer interface. The domain comprises a six-stranded beta sheet of the Rossmann fold. The central domain of Ku70 and Ku80 is a DNA-binding beta-barrel domain. The protein unique to the infected samples with the highest total peptide identifications was XRCC6 (P12956, XRCC6_HUMAN) which belongs to Ku70 family (UniProt KB). Anti-Ku70 antibody, PA1642, Western blotting. All lanes: Anti Ku70 (PA1642) at 0.5ug/ml.

Two of these proteins, Ku70 and Ku80, were identified by immunoblot analysis.

Tillsammans, Ku70 och Ku80 utgör Ku-heterodimeren , vilken binder till DNA- dubbelsträngbrott ändar och krävs för den icke-homolog sammanfogning (NHEJ)​ 

Validated for ELISA,IF,IHC,WB. Tested on 20 paraffin-embedded human tissues. UniProt (Universal Protein Resource) is the world's most comprehensive catalog of information on proteins created by joining the information contained in Swiss-Prot, TrEMBL, and PIR. UniProt builds upon the solid foundations laid by the Ku70 binding activity was localized to the C-terminal coiled-coil domain of nCLU.

Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non- homologous end joining (NHEJ) required for double-strand break repair.

KUB3 and KUB4 are unknown, >10 kb trans-cripts.

a) was performed using nuclear enriched cell extracts from the Ku70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). HSF1 interacted directly with both of the N-terminal sequences of the Ku70 and Ku86 proteins, which inhibited the endogenous heterodimeric interaction between Ku70 and Ku86. The blocking of the Ku70 and Ku86 interaction by HSF1 induced defective NHEJ repair activity and ultimately activated genomic instability after ionizing radiation (IR), which was similar to effects seen in Ku70 or Ku80 Invitrogen Anti-Phospho-Ku70 (Ser5) Polyclonal, Catalog # PA5-40247. Tested in Western Blot (WB) applications. This antibody reacts with Human, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL). Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair.
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KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. UniProt is an ELIXIR core data resource.

The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80.
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Ku70 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ku70 Antibody (PB9520) overnight at 4°C.

Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31)(Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), Hep G2 (Lane 5) and U-2 OS (Lane 6) were The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J Invitrogen Anti-Phospho-Ku70 (Ser5) Polyclonal, Catalog # PA5-40247. Tested in Western Blot (WB) applications. This antibody reacts with Human, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL).

Ku70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Ku70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Liu H., Herrmann C.H., Chiang K., Sung T.L., Moon S.H., Donehower L.A., Rice A.P. Positive elongation factor b (P-TEFb) is a cellular protein kinase that is required for RNA polymerase II (RNAP II) transcriptional elongation of protein coding genes. Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p1 (Ku70) and Xrcc5 (Ku80) in rat fibroblasts. Endoh D., Okui T., Kon Y., Hayashi M. Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-25915. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse samples. Supplied as 400 µL purified antibody (2 mg/mL). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8).

Knockdown of Ku70 was achieved by transfecting HeLa with Ku70 specific siRNAs (Silencer® select Product # S5455, S5457). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). Knockdown of Ku70 was achieved by transfecting HeLa cells with Ku70 specific siRNAs (Silencer® select Product # s5457, s5455). Western blot analysis (Fig.